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Plasmids were amplified by incubation of lysogeny broth (LB) cultures containing the appropriate antibiotics overnight at 37 C

EXPERIMENTAL MODEL AND SUBJECT DETAILS Mouse experiments This study was performed in strict accordance with all applicable federal and institutional policies

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20% uncertainty applies to all estimates

The capacity of your body to metabolize serotonin, which is a neurotransmitter that helps regulate mood and is found in your brain, is greatly impacted by the presence of vitamin B-12

This alone obfuscates anything you can hope to gleam from the study