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Typical cut-off levels for cotinine detection are: Urine: 200 ng/mL Saliva: 1014 ng/mL Blood: 10 ng/mL However, labs may adjust thresholds depending on the population and purpose (e.g., workplace, clinical, or insurance testing)

You must not have anything by mouth for 4 hours prior to your procedure , INCLUDING water, gum and hard candies
