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The procedure is based on the reduction of Ellmans reagent by SH groups to produce 5,5-dithiobis (2-nitrobenzoic acid) which has an intense yellow color that is measured spectrophoto- metrically at 412 nm using Thermo Scientific Genesis 10S spectropho- tometer

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Lactylation plays an essential role in liver diseases such as fibrosis, LI/R injury, and AILI, encompassing multiple biological mechanisms
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