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10.1186/s13018-019-1242-6 [DOI] [PMC free article] [PubMed] [Google Scholar] Petek M., Sikiric P., Anic T., Buljat G., Separovic J., Stancic-Rokotov D., et al

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The 72-hour threshold is not an official manufacturer guideline it's a practical working limit based on how quickly bacterial growth can become concerning at typical room temperatures (2025C) even in the presence of benzyl alcohol