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2005 Oct;26(10):1668-76

For cell permeability assay, brood size and vitellogenin assay, rigid strains ( p2 , p1p2 ) were initially grown on NGM plates and then supplemented with PA-loaded OP50 since they arrest as early larval stages in the presence of PA

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The emetic phase is characterized by a projectile, intense vomit, averaging 6 times/hour at the peak (first hour), often leading to significant dehydration (68)