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Peptide solutions were injected directly onto the analytical column (self-packed column, CSH C18 1.7 m beads, 300 m 30 cm) at a working flow rate of 5 L min 1 for 4 min

1-3, were seeded at 20,000 onto 35mm poly-L-lysine-coated glass-bottom dishes (Celvis) or coverslips in Neurobasal growth medium supplemented with 2% B27, 2 mM Glutamax, 50 U/ml penicillin, 50 g/ml streptomycin, and 5% fetal bovine serum (FBS) (Wang et al