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It is pesticide-free and BSE safe
After washing 3 times with 0.1 M PBS, 10 min each, the cells were incubated with corresponding secondary antibodies in blocking solution in the following composition: conjugated Alexa 647 donkey-anti-chicken anti IgG (1:500, 703605-155, Jackson), Alexa 488 donkey anti-guinea-pig IgG (1:500, 706545-148, Jackson), Alexa 488 donkey-anti-rabbit IgG (1:200, 711547-003, Jackson) for 2 h at room temperature with gentle rocking
2015 Jan 1;133(1):17-24
Subsequently, GluN1 expression was confirmed in the same anatomic sites of human myocardium [35], which can also express GluN2C [36] and GluN3 [37]
It is also used off-label, or without approval from the FDA, in the treatment of certain types of obesity-resistant to other interventions
