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At the beginning of the test, rats were gently placed in the center of the box and allowed to explore the arena freely for 5 min

The classical M1 activation phenotype, induced by the presence of IFN-, released by T cells, LPS or DAMP, is characterized by a microglia with the capacity to produce inflammatory cytokines (IL-23, IL-18, IL-12, IL-1, IL-6, TNF-, CCL2, and CXCL10), ROS, NO and matrix metalloproteinases (MMP9 and MMP3), as well as acting as an antigen-presenting cell preventing the entry of pathogens (Cherry et al., 2014

So far, imaging of Arabidopsis plants expressing either Grx1-roGFP2 or roGFP2-Orp1 has been mainly carried out using young seedlings grown in plates or leaf sections from mature soil-grown plants imaged by wide-field and/or confocal fluorescence microscopy as well as by the plate reader

Gene expression levels were calculated using the 2 -Ct method [ and expressed as relative units (RU) compared to the untreated controls where the level of gene expression was considered to be equal to 1

doi: 10.1038/s41598-019-44622-4 142 AlievaMMargaridoASWielesTAbelsERColakBBoquetaleCet al

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