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The cells were grown in DMEM containing 10% inactivated FBS and 1% penicillinstreptomycinamphotericin B and maintained in a humidified incubator at 37 C with 5% CO 2
View Abstract FDA guidance document on monitoring delayed adverse events a good first start

Furthermore, EGCG suppresses key signaling pathways such as the PI3K/AKT/mTOR and AMPK pathways, and also inhibits factors like ROS, iNOS, Cox-2, NF- B, Senescence-associated secretory phenotype (SASP), and p53-mediated cell cycle regulation in preadipocytes [558]

Antioxidants 12, 1375

Brisson BK, Spinazzola J, Park S, Barton ER
