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Phase II metabolism then further modifies these metabolites, resulting in the formation of conjugated metabolites that are more water-soluble and suitable for excretion

Ultraviolet (UV) radiation is identified as a major risk factor [33, 34]

For activation, a 50 l cofactor solution containing 0.9 mM glutathione, 0.1 mM hematin, and 0.24 mM TMPD (N, N, N, N-tetramethyl-p-phenylenediamine dihydrochloride) in 0.1 M Tris HCl buffer with a pH of 8.0 was added to the enzyme solution (10 l) and refrigerated on ice for five minutes

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A single colony was inoculated in Luria-Bertani medium (20 ml) with kanamycin (25 mg l-1) and cultured at 37 C overnight