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Pro-inflammatory microglia drive tau proliferation and toxicity by promoting neuroinflammation, such as activation of NLRP3 inflammasomes or induction of NF-kB signaling (23)

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doi: 10.1007/s11011-018-0348-6 409 MalarDSPrasanthMIBrimsonJMSharikaRSivamaruthiBSChaiyasutCet al

Cell Cycle 15 , 21642173 (2016)

Using a microplate reader at 488 nm excitation wavelength and 525 nm emission wavelength, the intensity of sample fluorescence was detected in real time, and changes in intracellular reactive oxygen species content were determined by comparing with the blank group ( 2.4 Lipid peroxidation assay Lipid peroxidation was determined by measuring the levels of malondialdehyde (MDA) as described in the Lipid Peroxidation MDA Detection Kit (Beyotime Biotech, China)

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